Fig. 2.

Partial stabilisation of opened RV cores in vitro by the addition of Mg²⁺ ions. Agarose gel 0.6%, electrophoresis with 10 mM MOPS/Tris buffer, pH 7.7, followed by silver staining. Lane 1: TLPs (partially degraded to DLPs); lanes 2 and 11: DLPs; lane 3: native RV cores; lane 4: cores + 400 μM EGTA; lane 5: cores + 400 μM EGTA + 1.6 mM MgCl₂ after 30 s; lane 6: cores + 400 μM EGTA + 1.6 mM MgCl₂ after 60 s; lane 7: cores +  [400 μM EGTA +1.6 mM MgCl₂] used simultaneously; lanes 8–10: reactions as described for lane 6 in the presence of NaCl 100 mM (lane 8), 200 mM (lane 9), and 400 mM (lane 10). All reactions were in 10 mM Hepes buffer, pH 7.4.