Fig. 3.

Formation of rotavirus DLPs and intermediates by addition of recombinant VP6 to cores opened by EGTA and restabilised by Mg^2+, spermidine³⁺, and cobalthexamine³⁺. Agarose gel 0.8%, electrophoresis with 10 mM MOPS/Tris buffer, pH 7.7, followed by staining with ethidium bromide. Lane 1: Purified DLPs; lane 2: purified cores: lane 3: cores + 400 μM EGTA; lane 4: cores + 400 μM EGTA + 1.6 mM MgCl₂ after 1 min; lane 5: cores + 400 μM EGTA + VP6; lane 6: cores + 400 μM EGTA + 1.6 mM MgCl₂ after 1 min + VP6; lane 7: cores + 400 μM EGTA + 300 μM spermidine³⁺ after 1 min; lane 8: cores + 400 μM EGTA + 300 μM spermidine³⁺ after 1 min; lane 9: cores + 400 μM EGTA + 350 μM cobalthexamine³⁺ after 1 min; lane 10: cores + 400 μM EGTA + 350 μM cobalthexamine³⁺ after 1 min + VP6; lane 11: 400 μM EGTA + 1.6 mM MgCl2 + VP6.