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. 2013 Dec 26;178(2):252–263. doi: 10.1016/j.virusres.2013.09.034

Fig. 7.

Fig. 7

Inhibition of packaging of radiolabelled ssRNA by cold ssRNA (basis for competitive packaging assay), Lane 1: DLPs; lane 2: native RV cores; lane 3: cores + EGTA; lane 4: as lane 3 + packaging mixture; as lane 4 + radiolabelled RV RNA7; lane 6: as lane 5 + RNase I; lanes 7–10: as lane 6, with unlabelled RV RNA added before packaging: 100 ng (7), 50 ng(8), 25 ng (9), 13 ng (10); lane 11: cores + EGTA + radiolabelled HIV-2 1104 RNA + packaging mixture + RNase I; lane 12: as lane 11, with 100 ng of unlabelled HIV-2 1104 RNA added before packaging. * Determined by densitometric analysis of scanned autoradiograph using the Image J programme ‘Image J’ (http://rsbweb.nih.gov/ij/).