Table 2.
Rescue of infectivity from rotavirus cores after various treatments in vitro followed by liposome-mediated transfection into KJ cells.
| Treatment of cores | Titration of yield (TCID50/ml) |
||
|---|---|---|---|
| Exp 1 | Exp 2 | Exp 3 | |
| None | Neg | Neg | Neg |
| EGTA | Neg | Neg | Neg |
| EGTA + Mg2+ | Neg | Neg | Neg |
| EGTA + VP6 | 100* | Neg | 2000 |
| EGTA + Mg2+ + VP6 | 1000* | 10,000 | 20,000 |
| EGTA + spermidine3+ | Neg | ND+ | Neg |
| EGTA + spermidine3+ + VP6 | 100 | ND | 20,000 |
| EGTA + cobalthexamine3+ | Neg | ND | Neg |
| EGTA + cobalthexamine3+ + VP6 | 1000 | ND | 5000 |
| None + Mg2+ + VP6 | ND | ND | 20,000 |
* Tested twice, + Not done.