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. 2013 Dec 5;9(12):e1003966. doi: 10.1371/journal.pgen.1003966

Figure 7. Cdk1– Clb2 inhibitory signal restrains MEN activation until anaphase.

Figure 7

(A) Premature Cdk1-Clb2 localization at the SPB during metaphase in the absence of Cdc55. Strains Y985 (MATa MET3-CDC20 CDC28F19 CDC14- myc9 Cdk1-eGFP BFA1-mCherry) and Y967 (as Y985, but cdc55Δ) were arrested in metaphase by Cdc20 depletion. At least 50 cells were scored for each strain. (B) Mob1 dephosphorylation upon Cdk1 inhibition. Strain Y1032 (MATa MET3-CDC20 CDC28-as1 GAL1-Flag3-ZDS1 MOB1-HA6) were arrested in metaphase by Cdc20 depletion and Zds1 was induced. After 180 min of Zds1 induction, 1 µM of 1NM-PP1 drug was added to inhibit Cdk1 to half of the culture and DMSO was added to the other half as a control. Scale bar, 2 µm.