Table 1. TbAS-A and TcAS-A kinetic parameters for aspartate, ATP and ammonia.

Species	Substrate	K m (mM)	V max ×10−3(mM.s−1)	k cat (s−1)	k cat/K m (M−1.s−1)
T. brucei	Aspartate	5.39±0.31	6.72±0.14	5.31±0.11	9.85×102
	ATP	1.80±0.32	7.88±0.48	6.22±0.38	3.46×103
	Ammonia	5.55±0.41	7.21±0.16	5.69±0.13	1.03×103
T. cruzi	Aspartate	6.45±2.05	3.09±0.40	2.41±0.32	3.74×102
	ATP	0.72±0.01	4.42±0.02	3.45±0.02	4.79×103
	Ammonia	7.75±1.55	3.14±0.21	2.45±0.17	3.16×102

The values are the means ± standard deviation obtained from 3 independent experiments.