Table 2.

Summary of miRNA analysis and engineering in CHO cells.

Experimental setting	Type of analysis	Outcome	Reference
Temperature shift	Microarray & qPCR	26 regulated miRNAs	Gammell (2007)
Transcription in recombinant cell lines	qPCR	16 miRNAs with de-regulation in recombinant DG44 cell lines	Lin et al. (2010)
Temperature shift	qPCR	10 regulated miRNAs (miR-7) miR-7 reduces growth and enhances qP	Barron et al. (2011a)
MicroRNA repertoire in various cell lines	NGS	380 conserved 22 novel miRNAs	Hackl et al. (2011)
MicroRNA repertoire in various cell lines	NGS	350 conserved miRNAs	Johnson et al. (2011)
Batch cultivation	Microarray & qPCR	118 miRNAs regulated during batch cultivation between lag, exponential and stationary growth phase	Bort et al. (2012)
Nutrient depletion & apoptosis	Microarray	70 miRNAs with regulation upon nutrient limitation	Druz et al. (2012)
MicroRNA overexpression screen	Engineering	miR-17 improves growth miR-21 reduces qP	Jadhav et al. (2012)
Transcription in recombinant cell lines	NGS	190 conserved microRNAs 93 microRNA regulated in two distinct recombinant cell lines	Hammond et al. (2012a)
Genomic context of microRNAs	In silico	Genomic annotation of 350 miRNAs	Hackl et al. (2012b)
Correlation to growth rate	Microarray	35 miRNAs with positive correlation to growth rate 16 miRNAs with negative correlation to growth rate	Clarke et al. (2012)
Specific microRNA knockdown	Engineering	miR-466h-5p knockdown improves batch performance of CHO cells	Druz et al. (2013)