Figure 1.
Schematic timeline summarizing dengue virus (DENV) challenge study and clinical sampling schedule. Sixteen DENV-seronegative rhesus macaques were divided into four groups, each consisting of two females (underlined numbers) and two males as indicated. Each group was inoculated subcutaneously with 105 plaque-forming units of DENV-1 (strain Western Pacific), DENV-2 (strain NGC), DENV-3 (strain Sleman/78), or DENV-4 (strain 814669), and clinical and antibody responses were evaluated over time. Blood samples were collected repeatedly over the first two weeks post-infection (asterisks), including before challenge (Pre) and on days 5, 6, 7, 8, 10, and 14 as indicated. Additional serum samples were collected on day 30 and at 30-day intervals thereafter until day 390.