Table 1. Evaluation of the 50% Inhibitory Concentration of soulamarin against trypomastigotes and intracellular amastigotes.
| compound | IC50 (µM) trypomastigotes (95%CI) | IC50 (µM) amastigotes (95%CI) | Hemolytic (%) activity at 1,300 µM | IC50 (µM) Cytotoxicity (95%CI) |
| Soulamarin | 219.8 (186.9–258.5) | 210.1 (174.5–252.6) | 0 | 278.3 (229.4–342.8) |
The viability of trypomastigotes was determined with MTT at 550 nm and the hemolytic activity was determined at 550 nm. The efficacy of soulamarin against intracellular amastigotes was determined using light microscopy counting.
95%CI – 95% confidence interval; IC50 – 50% inhibitory concentration. IC50 of benznidazole against trypomastigotes - 440.7 µM (95%CI 272.4–478.4 µM) and against intracellular amastigotes - 319.7 µM (95%CI 283.8–360.1 µM).