Figure 3. Neutrophil survival mediated by low oxygen levels, GCs, and GM-CSF.

(A) Percentage of neutrophil survival when cultured at 1% oxygen at 24 h (closed bars), 48 h (open bars), 72 h (cross-hatched bars), and 96 h (dotted bars) and cultured with or without 100 nM dexamethasone or 20 ng/ml GM-CSF. (B) Representative cytocentrifuge images, flow cytometry plots of AnnV/PI binding, and DNA integrity of 96-h GM-CSF-treated neutrophils, pre- and postcell sorting for viable cells (AnnV/PI-negative). (C) Representative histograms of aged, GM-CSF-treated, viable neutrophil response to fMLF (100 nM) with oxidative burst measured by the oxidation of DHR to rhodamine 123 and shape change assessed by change in forward-scatter. Data represent n = 6 and are shown as mean ± sem. ***P < 0.001.