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. 2013 Dec;94(6):1285–1292. doi: 10.1189/jlb.0912462

Figure 4. GCs attenuate GM-CSF-mediated neutrophil survival in hypoxic conditions.

Figure 4.

(A–C) The percentage of neutrophil survival when cultured with GM-CSF (20 ng/ml), with or without dexamethasone (100 nM) at (A) 21% oxygen, (B) 5% oxygen, or (C) 1% oxygen over a 96-h time-period; 24 h (closed bars), 48 h (open bars), 72 h (cross-hatched bars), and 96 h (dotted bars). Neutrophil survival in untreated cells under 1% oxygen levels over the 24-, 48-, 72-, and 96-h time-points are 69.4%, 31.1%, 17.4%, and 8.8%, respectively (data not shown). (D) Representative flow cytometry plots of AnnV/PI binding at 24 h, 48 h, 72 h, and 96 h in culture with GM-CSF (20 ng/ml), with or without dexamethasone (100 nM) at 1% oxygen. Data represent n = 6 and are shown as mean ± sem. *P < 0.05; **P < 0.01.

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