TABLE 2.

Primary Antibodies Used in This Study

Antigen	Host	Source	Cat. No./Lot No.	Dilution	Antigen
Calretinin	Goat	Millipore, Billerica, MA	AB1550/LV1486604	1:1,000	Rat calretinin
GFP	Goat	Rockland, Gilbertsville, PA	600-101-215/23153	1:1,000	GFP fusion protein corresponding to the full length amino acid sequence (246aa) derived from Aequorea victoria
PKC (clone MC5)	Mouse	Sigma, St Louis, MO.	P5704/097K4854	1:400	Amino acids 296-317 of PKC
Recoverin (Rec-1)	Rat	Gift from Dr. G. Adamus, Casey Eye Institute, Portland, OR		1:200	Purified recoverin (Adamus and Amundson, 1996)
Secretagogin	Rabbit	Gift from Dr Ludwig Wagner, University of Vienna, Austria		1:5,000	Recombinant human secretagogin (Wagner et al., 2000)
Synaptotagmin 2 (ZNP-1)	Mouse	Zebrafish International Resource Center, OR	ZNP-1/Prep date 02/10/09	1:50	1–5 day zebrafish embryo
VGLUT1	Guinea Pig	Millipore, Billerica, MA	AB5905/LV1439669	1:5,000	Synthetic peptide (aa 542-560 rat VGLUT1)