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A schematic of the light modulation system equipped with a UV LED as the light modulation source. A programmable motorized X-Y stage was used to spatially direct the modulated excitation light toward the sealed microwell of interest to determine the OCR via phase-based phosphorescence lifetime detection. (CL: collimating lens; BS: beam splitter; RS: reference signal; DS: detection signal; APD: amplified photo-detector; and PMT: photo-multiplier tube).
