Figure 7.

The metabolic profiles of a single developing zebrafish embryo at (a) 3 hpf and (b) 48 hpf, measured by sequentially adding pharmacological inhibitors of bioenergetic pathways. The time courses are annotated to show the relative contribution of non-mitochondrial respiration, respiration due to ATP turnover, respiration due to proton leak, maximal OCR after the addition of FCCP, and reserve capacity of a single developing zebrafish embryo. (oligomycin: inhibits ATP synthase; FCCP: an uncoupler that allows maximal respiration; and sodium azide inhibits total mitochondrial respiration) (Mean ± SEM; n > 8).