Figure 8.
(a) The variation in the components of the total basal respiration and the mitochondrial reserve capacity in pmol O2/min/embryo with developmental stage at 3, 7, 12, 24, 36, and 48 hpf. The total basal respiration is composed of a non-mitochondrial fraction and a mitochondrial fraction, which is further composed of the parts of respiration associated with proton leak and with ATP turnover. (b) The change in the partitioning of the total basal respiration with zebrafish embryonic development, determined by calculating the percentage that each fraction of respiration contributes to the total basal respiration (100%). (Mean ± SEM; n = 4 from 2 × 2 microwells).