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. 2013 Dec 5;9(12):e1003979. doi: 10.1371/journal.pgen.1003979

Figure 3. 2D neutral/alkaline gel analysis of Alu-IR-induced DSBs in theTET-POL3Δsae2 strain.

Figure 3

Yeast DNA embedded in agarose plugs was digested with AflII (A) or BglII (B). Digested DNA was separated in neutral conditions in the first dimension. In the second dimension, DNA was run in either neutral (left gel) or alkaline (right gel) conditions. Southern hybridization was done as described in Figure 2.