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. Author manuscript; available in PMC: 2013 Dec 6.
Published in final edited form as: Cell Rep. 2013 Jun 27;4(1):10.1016/j.celrep.2013.05.041. doi: 10.1016/j.celrep.2013.05.041

Figure 5. Investigating the Relationship of the Checkpoint-Kinase-Dependent Transcriptional Response with the Cell Cycle.

Figure 5

(A) Checkpoint-kinase-dependent genes with peak expression in G1 phase are upregulated in response to MMS treatment, whereas genes with peak expression in S, G2, and M phases are downregulated. Graph shows p values for enrichment of genes reported to have peak expression at different stages of the cell cycle (Spellman et al., 1998) among the upregulated and downregulated sets of Rad53-dependent genes.

(B) Applying the same enrichment analysis for cell-cycle-specific expression to Rad53-dependent genes that are predicted targets of the TFs shown in Figure 3C reveals that upregulated targets of Mbp1, Swi4, Swi6, Fkh2, and Ndd1 are enriched for G1 genes, downregulated targets of Fkh2 are enriched for S/G2 genes, and downregulated targets of Swi4, Swi6, Fkh2, Mcm1, and Ndd1 are enriched for G2/M genes (Table S9).

(C) The Rad53-dependent transcriptional regulatory network for predicted targets of Mbp1, Swi4, and Swi6.

(D) FACS analysis of asynchronous cultures of untreated and MMS-treated WT, rad53Δ, mec1Δ, and dun1Δ strains (see Figure S6 for other strains). See also Tables S4 and S9 and Figure S6.