Table 2. Readouts from proliferation/mitotic imaging data.
Readout | Description |
Cell Count | The total number of cells (nuclei) stained with Hoechst 33342 and detected in the DAPI channel |
EdU positive cells | The percentage of actively proliferating cells that have average FITC (EdU) intensity above the EdU threshold |
Mitotic cells | The percentage of cells that are MPM2-positive |
Non-mitotic cells that are EdU-positive | The percentage of EdU-positive cells within the non-mitotic population |
Mitotic cells that are EdU-positive | The percentage of EdU-positive cells within the mitotic population |