Figure 1. DEX particles are of spherical appearance and uniform in size.

(A) Shape and size distribution of DEX(−) dispersed in PBS was studied by electron microscopy. (B) Hydrodynamic radii of Dextran T500 and derived DEX particle formulations as function of q² in DPBS buffer (0.33 mg/ml) were determined by DLS (see Methods). Graphs denote the angular dependency of the apperent diffusion coefficient of the different dextran solutions in buffer solution. (C) To assess cytotoxic effects of DEX particles on BM-DC viability, cells (2.5×10⁵) were seeded into wells of 96 well cell culture plates in a volume of 50 µl in triplicates, and DEX(−) particles (20 mg/ml) were added at different amounts as indicated. One day later, viability of BM-DCs was assessed as described (see Methods). The viability of untreated BM-DCs was set to 100% (dashed line).