Table 3.
Level of Nitrated Proteins from Old SAMP8 Mice Injected with AO against PS-1 (C) Compared to Old SAMP8 Mice Treated with Water (A) or Random AO(B)
| Spot | Protein Identified | Accession # | Coverage | Number of identified peptides (a) | Score | MW (kDa) | pI | Groups | P value (c) | Fold (b) |
|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Isoform 2 of Glial fibrillary acidic protein (GFAP) | P03995-2 | 30.84 | 14 | 67.67 | 49.3 | 5.50 | A-C | 0.027 | 15.4 ↑A |
| 2 | Peptidase (Mitochondrial processing) alpha (DPL2) | A2AIW8 | 8.02 | 4 | 13.37 | 58.2 | 6.83 | B-C | 0.037 | 3.79 ↑C |
| 3 | Dihydropyrimidinase-related protein 2 | O08553 | 32.52 | 14 | 103.38 | 62.2 | 6.38 | B-C | 0.047 | 9.5 ↑C |
| 4 | Isoform 5 of Dynamin-1 (DYN1) | P39053-5 | 22.91 | 18 | 91.49 | 96.0 | 7.01 | B-C | 0.04 | 2.79 ↑B |
| 5 | Vesicle-fusing ATPase/Vesicular-fusion protein NSF (NSF) | P46460 | 26.08 | 18 | 86.01 | 82.6 | 6.95 | A-B | 0.048 | 3.85 ↑B |
| 6 | Dihydropyrimidinase-related protein 1 (CRMP-1) | P97427 | 7.17 | 3 | 9.26 | 62.1 | 7.12 | B-C | 0.018 | 3.37 ↑B |
| 7 | Isoform 3 of Septin-11 (SEP11) | Q8C1B7-3 | 22.35 | 9 | 48.34 | 48.9 | 6.81 | B-C | 0.0072 | 5.24 ↑B |
| 8 | Alpha-enolase (ENO) | P17182 | 21.43 | 7 | 28.71 | 47.1 | 6.8 | A-B | 0.04 | 7.14 ↑B |
| 9 | Actin-related protein 2 (Actr2) | P61161 | 27.41 | 9 | 47.52 | 44.7 | 6.74 | B-C | 0.047 | 1.88 ↑B |
| 10 | L-lactate dehydrogenase B chain (LDHB) | P16125 | 8.08 | 2 | 9.00 | 36.5 | 6.05 | B-C | 0.047 | 3.79 ↑B |
| 11 | Isoform 2 of Isocitrate dehydrogenase (NAD) subunit alpha, mitochondrial (IDH3A) | Q9D6R2-2 | 7.64 | 2 | 4.96 | 31.4 | 6.04 | A-C | 0.036 | 42.52 ↑A |
a
The number of peptide sequences identified by nanospray ESI-MS/MS of tryptic peptides.
b
The fold-change in spot density of (C) compared with (A) and (B). The arrow indicates the direction of change.
c
The p-value associated with fold-change calculated using a Student’s t-test.