Figure 6. Frequency of Amino Acid appearance in the include/exclude metabolite lists for S. oneidensis.

While twelve amino acids were not included in the original de novo biomass proposals of BioMog (Table 1), the growth phenotypes do not preclude their addition as shown here. Their exclusion by BioMog is a result of insufficient evidence for their inclusion based soley on the growth phenotype experiments performed. Metabolites in green are those that were selected by BioMog as essential biomass components. Metabolites in blue are those that have evidence to support their inclusion but were omitted because they are redundant with other metabolites for matching experimental results (e.g., isoleucine, leucine and valine biosynthesis pathways share many of the same enzymes). Metabolites colored red have no growth phenotype evidence to support their inclusion or exclusion from the biomass.