Table 2. Primers and probe sequences used in qPCR screen of canine stool samples for enteric viruses.
Virus | Primer/Probe | Ref |
Canine norovirus | F: GCTGGATGCGGTTCTCTGACR: TCATTAGACGCCATCTTCATTCACProbe: FAM-AGCGAGATTGCGATCTCCCTCCCACAT-BHQ | This report |
Canine parvovirus | F: AAACAGGAATTAACTATACTAATATATTTAR: AAATTTGACCATTTGGATAAACTProbe: VIC-TGGTCCTTTAACTGCATTAAATAATGTACC-BHQ | [32] |
Canine entericcoronavirus | F: TTGATCGTTTTTATAACGGTTCTACAAR: AATGGGCCATAATAGCCACATAATProbe: Cy5-ACCTCAATTTAGCTGGTTCGTGTATGGCATT -BHQ | [32] |
Equine arteritis virus(internal control) | F: CATCTCTTGCTTTGCTCCTTAGR: AGCCGCACCTTCACATTGProbe: Cy5.5-CGCTGTCAGAACAACATTATTGCCCAC-BHQ2 | [33] |
Four primer-probe sets were used in the viral nucleic acid survey. CPV, CECoV and EAV primer-probe sets have previously been published whereas the CNV primer-probe set was designed for this study.