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HSR‐GBM1B neurospheres and differentiation induced by serum. (A) Phase‐contrast microphotograph of HSR‐GBM1B neurospheres grown in serum‐free, epidermal growth factor and fibroblast growth factor supplemented medium for 3 days. (B) Morphology change of neurosphere cells after serum treatment. C–H Expression of the neuronal marker Tuj1 (C,D), the astroglial marker GFAP (E,F) and the oligodendrocyte marker GalC (G,H) in HSR‐GBM1B cells after serum‐induced differentiation. Bar = 15 μm.
