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. 2012 Apr 4;103(6):1028–1037. doi: 10.1111/j.1349-7006.2012.02260.x

Figure 2.

Figure 2

Inhibitor of DNA binding protein 2 and 4 (Id2 and Id4) gain‐of‐function induces neuronal/astroglia differentiation while suppressing oligodendrocyte differentiation. (A) HSR‐GBM1B neurospheres were transfected with control plasmid (Con), Id2 cDNA (pId2) or Id4 cDNA (pId4) for 72 h. The expression of Id2 or Id4 protein was increased approximately 3.3 and 6.7‐fold, respectively. (B) In cells transfected with Id2 or Id4 cDNA, increased level of Id2 or Id4 induced the expression of neuronal marker Tuj1 and astroglial marker GFAP in HSR‐GBM1A, HSR‐GBM1B and Mayo 39. (C) Immunofluorescent staining confirmed that GFAP and Tuj1 were increased by overexpression of Id2 or Id4 in HSR‐GBM1B and Mayo 39 cells. In contrast, expression of oligodendrocyte marker GalC was decreased following Id2 or Id4 overexpression. (D) GalC expression in untransfected (WT) and control transfected HSR‐GBM1A cells indicating that transfection did not affect GalC expression. (E) Quantitative RT‐PCR of GalC expression confirmed that Id2 or Id4 overexpression suppressed GalC expression (n = 3). **P < 0.01. Bar = 15 μm.