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. 2012 Apr 4;103(6):1028–1037. doi: 10.1111/j.1349-7006.2012.02260.x

Figure 3.

Figure 3

Inhibitor of DNA binding protein 2 or 4 (Id2 or Id4) overexpression reduces stem cell marker expression and inhibits neurosphere formation. (A, B) HSR‐GBM1B and HSR‐GBM1A were transfected with Id2 or Id4 cDNA and stable line were selected. Expression of stem cell markers Msi1, Sox2 and nestin was quantified by quantitative RT‐PCR. In HSR‐GBM1B cells, Id2 induction inhibited expression of Msi1, Sox2 and nestin by 28%, 28% and 44%, respectively. In HSR‐GBM1A cells, Id2 or Id4 induction also reduced expression of all three markers tested (n = 4). (C) Overexpression of Id2 or Id4 reduced the side population in GBM neurosphere cultures (n = 4). (D) Glioblastoma multiforme (GBM) neurosphere cells were transfected with Id2 or Id4 cDNA for 72 h and grown for 7–10 days. Id2 or Id4 induction significantly inhibited neurosphere formation in four different GBM neurosphere cultures (n = 3), suggesting that Id2 and Id4 depleted GBM stem cell pools. *P < 0.05; **P < 0.01.