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. 2013 Dec 5;8(12):e81574. doi: 10.1371/journal.pone.0081574

Figure 2. Data analysis strategy used to identify and quantify the circulating endothelial cells.

Figure 2

Our data analysis gating strategy assures the identification and quantification of the circulating endothelial cells (CEC) and endothelial progenitor cells (CEP), among stored CD146+ cells. Using the Paint-a-Gate™ or the Infinicyt™ software we first identified the beads (blue dots) in the CD45 vs. CD146 dot plot (A); after that, we selected the total CD146+ events (green dots) (B, for selection of CD146+ cells; C, showing the light scatter properties of the cells); after excluding the CD146- cells (D to F), we finally were obtained the CEC (CD45-CD146+, yellow dots), the CEP (CD45+lowCD146+, red dots), activated lymphocytes (CD45+highCD146+, green dots), as well as the beads (blue dots), being then able to perform CEC and CEP quantification.