Table 1. Characteristics of the monoclonal antibodies used in this study.
| Cluster designation | Clone | Fluorochromes | Supplier | Specificity | Expression on endothelial cells |
| CD45 | 2D1 | PerCP | BD Bioscience | Leukocyte Common Antigen (LCA) | Progenitor* |
| CD54 | HA58 | APC | BD Pharmingen | Intercellular Adhesion Molecule 1 (ICAM-1) | Activated |
| CD62E | BBIG-E5 (10C10) | FITC | R&D Systems | E-Selectin | Activated |
| CD133 | AC133 | APC | Miltenyi Biotec | Prominin 1 (PROM1) | Progenitor |
| CD142 | VD8 | FITC | American Diagnostica | Tissue factor | Activated |
| CD146 | P1H12 | PE | BD Pharmingen | Melanoma cell adhesion molecule (MCAM or Mel-CAM) | All |
Abbreviations: PerCP, peridin-chlorophyll protein; APC, allophycocyanin; FITC, fluorescein isothiocyanate; PE, phycoerythrin.
*
CD45 has been described as being absent on mature endothelial cells and expressed at very low levels on endothelial progenitor cells.