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. 2013 Dec 5;8(12):e81504. doi: 10.1371/journal.pone.0081504

Figure 2. The fluorescence-based thermal shift assay is tractable for use with MAP2K4.

Figure 2

A. MAP2K4 undergoes a two-phase transition denaturation profile. MAP2K4 was subjected to FTS analysis, as described in Methods, in the presence of 1 mM ATP, 1 mM L-glycine, or buffer only. Depicted is a representative denaturation profile. B. The effect of adenine moiety containing ligands upon the denaturation profile of MAP2K4. Experiment was performed as in A in the presence of the indicated ligands, all at 1 mM, and the resultant ΔTm values, compared to control (HEPES), determined. Values shown are the mean ± S.D (n = 3).