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. Author manuscript; available in PMC: 2013 Dec 7.
Published in final edited form as: Plant J. 2004 Nov;40(3):10.1111/j.1365-313X.2004.02218.x. doi: 10.1111/j.1365-313X.2004.02218.x

Figure 3.

Figure 3

Molecular analysis of transgenic plants showing a range of light hyper-responsive phenotypes.

(a) Northern blot analysis of TDET1 mRNA levels in leaves of transgenic plants with different phenotypes.

(b) Northern blot analysis of TDET1 and CAB mRNA levels in fruits of transgenic plants with different phenotypes.

(c) RT-PCR analysis of fruits from transgenic plants showing different phenotypes. Full-length TDET1 was synthesized using specific primers and HY5 primers were used as a control for cDNA synthesis and loading.

(d) Northern blot analysis of TDET1 mRNA levels in fruits from the same truss. Severity of light hyper-responsive phenotypes correlated negatively with expression levels of TDET1.

In (a), (b) and (d) 28S rRNA is shown as loading control. MM plants containing the 35S::hp2 construct were used for all analyses. Wild-type (WT), Blotchy (Bl) and high pigment (hp) fruit phenotypes.