Skip to main content
. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Arterioscler Thromb Vasc Biol. 2013 Sep 5;33(11):10.1161/ATVBAHA.113.302054. doi: 10.1161/ATVBAHA.113.302054

Figure 5. Increase in mRNA and decrease in protein expression of BMPRs in human lung tissues from HIV infected IVDUs.

Figure 5

A. Total RNA was extracted from frozen human lung tissues from HIV-infected individuals with (HIV+IVDU group, n=4) or without IVDU (HIV group, n=4) and uninfected individuals with (IVDU group, n=3) or without IVDU (normal group, n=3). Quantitative analysis of BMPR-2,-1A and -1B was carried out by Real-Time RT-PCR using the SYBR Green detection method. Mean+ SD, *p<0.05 compared to normal, #p<0.05 compared to IVDU, $p<0.05 compared to HIV group. B. Expression of BMPR-2, -1A and -1B was analyzed by Western blot of total protein extract obtained from frozen lung tissues. Upper panel shows the western blot image and lower panel represents the densitometric analysis of the immunoblots. Mean+ SEM, *p<0.05 compared with normal, #p<0.05 compared with IVDU, $p<0.05 compared with HIV. C. Representative photomicrographs of BMPR-2 immuno-histochemistry on paraffin-embedded lung sections from Normal, HIV+/-IVDUs are shown. Original magnification 100X, Scale bar: 100μm.