Fig. 3.

Biological and functional characteristics of mcDC. a identification of mcDC within the CD11c^high population. Upon gating for CD11c^high and exclusion of pDC by PDCA-1, mcDC are found in the CD8⁻CD11b^−/low population. b Particle size of phagocytosed apoptotic material. Indicated DC populations were incubated with Violet Trace-labeled apoptotic cells and particle size was determined 20 h later using Amnis Imagestream. c Prolonged Ag presentation by mcDC. Indicated DC populations were incubated with OVA-expressing MHC class I deficient irradiated tumor cells. After 20 h, DC were purified and an OVA-specific CD8⁺T cell hybridoma was added after an additional 24, 48, or 72 h. mcDCs were the only DCs that showed activation of the T cell hybridoma. Viability of the different DC populations was comparable indicating that mcDC have a distinct capacity for persistent Ag presentation