Fig. 3.

Effect of U19/EAF2 knockout on the expression of BRAF mRNA and p-ERK in the mouse model. a. qPCR analysis of the BRAF mRNA levels in anterior prostate (AP) and ventral prostate (VP) and b. lung and liver of U19/EAF2 knockout and wild-type control mice.. Data were normalized to GAPDH, (n = 3). c. Western blot analysis of phosphorylated ERK, pERK(p44/42), in U19/EAF2 knockout and wild-type mouse tissues. Images shown are representative blots for ERK, pERK and GAPDH from ventral prostates and liver lysates of individual U19/EAF2 knockout and wild-type control mice. d. Intensity of p-ERK in /EAF2 knockout and wild-type mouse tissues. Band densities in the Western blots were analyzed with IMAGEJ (National Institutes of Health) and normalized against GAPDH. Data represent the mean ± SEM; * p < 0.05. ** p < 0.01, *** p < 0.001 versus WT