Fig. 1.
Lumican is an endogenous inhibitor of tumor growth. a PCR based genotyping. PCR amplified 190 bp Lumican product in wild type (WT) mouse and 390 bp mutant DNA product in Lumican knockout (KO) mouse. PCR amplified both 190 bp Lumican product and 390 bp mutant DNA product in heterozygous (HET) mouse. b 1 × 106 Pancreatic Adenocarcinoma (PanO2) cells were injected subcutaneously in triplicate into WT, HET, and KO mice. One week after injection, tumors were detectable and tumor size was measured every other day. c Representative tumors from a single experiment isolated from mice after 15 days. d Bar graph representing final tumor weights from WT, HET, and KO mice. Each data point represents Mean ± SD. * indicate P < 0.05, Student T-test, N = 3