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. Author manuscript; available in PMC: 2014 Nov 1.
Published in final edited form as: Biochem Biophys Res Commun. 2013 Aug 22;440(4):10.1016/j.bbrc.2013.08.031. doi: 10.1016/j.bbrc.2013.08.031

Figure 2. RNF38 is capable of autoubiquitination in vitro.

Figure 2

Reactions contained recombinant RNF38, E1, HA-ubiquitin, and either UbcH2, UbcH5b or UbcH7 with or without 1mM ATP. Purified GST was used as a control. Reactions were analyzed by immunoblot with anti-RNF38 (sc-102096) and anti-HA antibodies.