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. 2013 Dec 5;8(12):e82237. doi: 10.1371/journal.pone.0082237

Figure 4. Activation of human somatostatin receptor subtype-5 (hSSTR5) by membrane-tethered somatostatin.

Figure 4

Yeast strains IMFD-72 and IMFD-72ZsD, which coexpress pGK421-SSTR5 and either pGK426-S1442 (SST) or pGK426-alpha42 (α-factor), were incubated in pH-adjusted SD selective medium. (A) The GFP fluorescence of 10,000 cells was measured by flow cytometry. Mean values of the green fluorescence signal of 10,000 cells. Error bars represent the standard deviations (n = 3); ***, p < 0.001, by one-way ANOVA, Tukey’s post test. (B, C) Fluorescence microscopy analysis of cells incubated for 9 h. (B) Cells were examined using the 40× objective lens of a fluorescence microscope. Exposure time was 0.67 s. The left photographs are fluorescence micrographs, and the right photographs are bright-field micrographs. (C) Cells were examined using the 100× objective lens of a fluorescence microscope. Exposure time was 0.14 s.