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. 2013 Dec 5;8(12):e82237. doi: 10.1371/journal.pone.0082237

Figure 9. Activation of human neurotensin receptor subtype-1 (hNTSR1) by membrane-tethered neurotensin.

Figure 9

(A) Amino acid sequences of membrane-tethered peptides. (B, C) Yeast strain IMFD-72ZsD, which coexpresses pGK421-NTSR1 and either pGK426-NTS42 (NTS), pGK426-NTS(8-13)42 (NTS(8-13)), pGK426-NMN42 (NMN) or pGK426-alpha42 (α-factor), was incubated in pH-adjusted SD selective medium. (B) The GFP fluorescence of 10,000 cells was measured by flow cytometry. Mean values of the green fluorescence signal of 10,000 cells. Error bars represent the standard deviations (n = 3); *, p < 0.05, and ***, p < 0.001, by one-way ANOVA, Tukey’s post test. (C) Fluorescence microscopy analysis of the cells incubated for 24 h. Cells were examined using the 40× objective lens of a fluorescence microscope. Exposure time was 0.67 s. The left photographs are fluorescence micrographs, and the right photographs are bright-field micrographs.