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. Author manuscript; available in PMC: 2014 Oct 24.
Published in final edited form as: Cell. 2013 Oct 24;155(3):10.1016/j.cell.2013.09.023. doi: 10.1016/j.cell.2013.09.023

Figure 4. FinMajor has a defect in nuclear poly(A)+ RNA export.

Figure 4

(A) hGLE1 siRNA treatment depletes endogenous hGle1 protein levels. Immunoblot analysis of hGle1 and actin protein levels in scrambled control (CTRL) or hGLE1 siRNA-treated HeLa cells transfected with the indicated GFP-tagged proteins. (B) Nuclear poly(A)+ RNA accumulation in hGLE1 siRNA treated cells expressing the indicated GFP-tagged proteins, detected by in situ oligo-dT hybridization and direct fluorescence microscopy. See Figure S3B for quantification of the nucleocytoplasmic distribution of poly(A)+ RNA.