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. 2013 Dec 5;8(12):e82054. doi: 10.1371/journal.pone.0082054

Figure 4. Validation of predicted ssc-miR-147 target genes.

Figure 4

An RCAS-based miRNA expression was utilized to authenticate selected predicted miR-147 target sites. The hairpin for ssc-miR-147 was cloned into RCASBP(A)-YDV using Gateway cloning (Life Technologies). DF1cells ectopically expressing miR-147 or a scrambled control (SC) were transfected with psiCHECK2 constructs containing miR-147 binding sites cloned downstream of the Renilla luciferase gene. Relative Renilla luciferase activity is shown. *: p<0.05, **: p<0.01. aThis construct contains one miR-147 binding site. bThis construct contains two miR-147 binding sites.