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. 2013 Dec 5;8(12):e82054. doi: 10.1371/journal.pone.0082054

Figure 5. Over expression of miR-147 in PRRSV infected PAMs using a synthetic mimic.

Figure 5

PAMs were transfected with 100miR-147 mimic (Dharmacon) or a negative control mimic (Dharmacon) using amine-based transfection. Sixteen hours post-transfection, PAMs were infected with PRRSV (VR-2332) at M.O.I. 1. A. Real-time RT-PCR analysis of miR-147 expression in PAMs transfected with either the miR-147 mimic (grey bars) or the negative control mimic (black bars). ***p≤0.001. B. Real-time RT-PCR analysis of Snapin, a miR-147 target, in PRRSV-infected PAMs transfected with a miR-147 mimic (grey bars) or a negative control mimic (black bars). *p≤0.05, **p≤0.01.