Skip to main content
. 2013 Dec 5;8(12):e82054. doi: 10.1371/journal.pone.0082054

Figure 6. PRRSV N levels in PAMs transfected with either a miR-147 mimic or a negative control mimic.

Figure 6

PAMs were transfected with 100miR-147 mimic (Dharmacon) or a negative control mimic (Dharmacon) using amine-based transfection. Sixteen hours post-transfection PAMs were infected with PRRSV (VR-2332) at M.O.I. 1. Total RNA was isolated at 8 hpi, 12 hpi and 24 hpi. Real-time RT-PCR analysis of the PRRSV nucleocapsid protein mRNA levels in PAMs transfected with either a miR-147 mimic (grey bars) or a negative control mimic (black bars). *p≤0.05, **p≤0.01.