Table 3. Computationally predicted porcine miR-146a target sites selected for validation.
Symbol/GI | mRNA binding sitea | Score/Energy (kcal/mol) | Location |
IL1A/1987 | 5′ aTGCTTCT--TGAAGCTGAGCCt 3′ | 165/-23.1 | 1493–1513 |
5′ taGTCACTCGCTGAGCATGTGCTGAGCCt 3′ | 154/-22.8 | 931–959 | |
IL1B/47522925 | 5′ cccaTCCTCAGGCCCCATCCACTGAGCCA 3′ | 153/-21 | 1061–1089 |
IL13/189176150 | 5′ gatTTCCTTAGCTTAGACTTGAGCCt 3′ | 151/-18.9 | 581–606 |
IRG6/47523213 | 5′ ggGGTGCTGTGTGAATCTCCTGAGTCt 3′ | 142/-18.1 | 1737–1763 |
5′ aTG-TCAT-TTGAACTGGGTCA 3′ | 140/-22.2 | 2058–2077 | |
5′ CTGATGAGTCTGAAGCCCAAATCCTGAGTCA 3′ | 135/-17.6 | 2683–2713 | |
GBP1/166202345 | 5′ CTG----TGCTTCACTGAGCTt 3′ | 144/-17.8 | 2956–2973 |
IL12A/47522811 | 5′ gTGTT--TGTAGAAACAAACACTTGAGCCt 3′ | 136/-20.1 | 1248–1275 |
CD4/47641126 | 5′ CTG----TGCTTCACTGAGCTt 3′ | 138/-18.6 | 2652–2673 |
CD80/55742787 | 5′ CTCTCACAGTAGTGAGAATTCTGAGCCc 3′ | 146/-19.7 | 1606–1636 |
ICAM1/55742637 | 5′ CTGTAGCCACAGCACTGAGCCA 3′ | 167/-21.7 | 1855–1876 |
CCR1/44890871 | 5′ ggcTTCTAGTTGTACGGCTGAGCgt 3′ | 139/-16.8 | 2398–2422 |
Capitalized text indicates basing pairings with the microRNA.