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. Author manuscript; available in PMC: 2013 Dec 7.
Published in final edited form as: J Steroid Biochem Mol Biol. 2009 Mar 3;114(0):10.1016/j.jsbmb.2009.02.009. doi: 10.1016/j.jsbmb.2009.02.009

Fig. 2.

Fig. 2

Immunoblot analysis of SMAD3 expression in liver cytosol from CFTRΔF508 mice. Cytosol (200 mg) prepared from livers of female CFTRΔF508 of each genotype (+/+, +/−, −/−) was resolved in a 12% SDS-polyacrylamide gel, electrotransferred to nitrocellulose membranes, immunoblotted with a rabbit anti-SMAD3 antibody (Calbiochem) and developed with the Pierce West Pico ECL substrate. Panel A shows the immunoreactivity of SMAD3 from two each female CFTRΔF508(+/+, +/−, −/−) mice. Panel B shows the levels of SMAD3 and SULT1E1 activity in liver cytosols of different genotype CFTRΔF508 mice.