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. Author manuscript; available in PMC: 2013 Dec 7.
Published in final edited form as: J Steroid Biochem Mol Biol. 2009 Mar 3;114(0):10.1016/j.jsbmb.2009.02.009. doi: 10.1016/j.jsbmb.2009.02.009

Fig. 4.

Fig. 4

Quantitative RT-PCR analysis of SULT1E1 expression in HepG2 cells co-cultured with MMNK-1 cells. HepG2 cells were co-cultured with CFTR-siRNA or control-siRNA MMNK-1 cells for increasing times then total RNA was extracted and utilized to synthesize cDNA. Quantitative RT-PCR was performed with TaqMan® Gene Expression Assays for human SULTs 1E1, 2A1 and 1A1. Ribosomal 18S RNA was chosen as the endogenous control for total RNA normalization and mRNA expression levels were calculated using the Ct method, where the calibrators were the samples from normal HepG2 cells without co-culture with MNNK-1. Relative expression of SULT isoforms was presented as fold-change between co-culture with CFTR-siRNA and co-culture with control-siRNA. Each point represents the mean of four separate determinations.