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. Author manuscript; available in PMC: 2013 Dec 7.
Published in final edited form as: J Steroid Biochem Mol Biol. 2009 Mar 3;114(0):10.1016/j.jsbmb.2009.02.009. doi: 10.1016/j.jsbmb.2009.02.009

Fig. 6.

Fig. 6

Induction of SULT1E1 in HepG2 by LXR ligands. HepG2 cells were treated with TO901317, 24(S)-hydroxycholesterol, desmosterol and 7-dehydrocholesterol at varying concentrations for 48 h. Cytosol was prepared from the cells and SULT1E1 and SULT2A1 activities were determined using 20 nm [3H]-E2 or 4 μM [3H]-DHEA as substrate, respectively, with 25 μM PAPS as the sulfate donor as described previously [14]. SULT1A1 activity was assayed with 4 μM p-nitrophenol and [35S]-PAPS as described previously [43].