Table 3.

Proteomic analysis of cytosolic protein changes in male CFTR KO and AF508 mice. Cytosolic protein expression in a male KO(−/−) mouse (SULT1E1 13.74, pmol/min/mg) was compared to that of a littermate control (0.26 pmol/min/mg). In a separate experiment, cytosolic protein expression in a male AF508(−/−) mouse (16.1 pmol/min/mg) was compared to that of a littermate control (0.97 pmol/min/mg). Cytosol (100 μg) from each liver was focused in a Bio-Rad Criterion system using a 4–7 pH gradient and processed as describe above—duplicate gels were run for each sample and analyzed for trends in changing intensities of protein spots comparing the KO or AF508 to its control. In the ΔF508 mice, two control proteins whose intensities were not different were included.

KO Mice			ΔF508 Mice
Spot	KO/Contrd@	Identity	Spot	ΔF508/Contrd@	Identity
3401	8.15	Apolipoprotein A IV	0001	0.31	Unknown
5501	5.6	Met Adenosyltrans.	0012	*	Major urinary protein
706	0.24	Albumin	5005	2.0	Adenosine kinase
9201	*	Glutathione S-transferase M1	4001	*	Fatty acid binding protein#
1102	0.02	Major urinary protein	3404	⊗	Regucalcin
4706	3.1	Heat shock protein	6001	6.1	Fatty acid binding protein#
2504	3.15	ATP synthase	4	*	Apolipoprotein E
2304	0.34	Regucalcin	3202	0.17	ApolipoproteinA1#
9203	*	Carbonic anhydrase	3203	0.38	Apolipoprotein A1#
10	*	Glutathione S-transferase P1	3713	Control	Heat shock protein
1002	3.13	Paralbumin	3506	Control	Actin

^*No protein was detectable in the KO or ΔF508 but was detectable in the controls.

^⊗A protein modification occurred that affected gel migration.

^#These proteins are related isoforms.