Table 4.
Pathway analysis of cholesterol and sterol metabolism in Human Gene 1.0 array data. Total RNA was isolated from control and CFTR-siRNA-MMNK-1 cells and used for expression profiling using the Human Gene 1.0 ST array. Both control and siRNA arrays were done in triplicate. Genes showing a significant difference in expression at p < 0.05 were used for further analysis. After statistical analysis, the resulting list (p < 0.05) was loaded into PathwayArchitect software (Agilent, CA, USA) for Gene Ontology (GO) analysis. Among 1775 probe sets matched to GO database of 90,204 total GO terms, 20 probe sets relate to cholesterol metabolism, e.g., with a p-value of 7.45E–16 when the two ratios of the same term (cholesterol metabolism) were compared.
| GO Term | # Genes in selection | Rows in selection | # Genes in database | Total rows in database | p-value |
|---|---|---|---|---|---|
| Cholesterol biosynthesis | 14 | 1775 | 33 | 90204 | 7.17 E–16 |
| Cholesterol homeostasis | 2 | 1775 | 7 | 90204 | 0.007 |
| Cholesterol metabolism | 20 | 1775 | 89 | 90204 | 7.45 E–16 |
| Cholesterol transport | 2 | 1775 | 10 | 90204 | 0.016 |
| Sterol biosynthesis | 14 | 1775 | 49 | 90204 | 1.79 E–14 |
| Sterol metabolism | 21 | 1775 | 100 | 90204 | 6.18 E–16 |