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. Author manuscript; available in PMC: 2014 Aug 29.
Published in final edited form as: Neuroscience. 2013 May 3;246:10.1016/j.neuroscience.2013.04.059. doi: 10.1016/j.neuroscience.2013.04.059

Fig. 4.

Fig. 4

Locomotor activity after cocaine-induced behavioral sensitization in WT, ASIC1a−/−, and ASIC2−/− mice. Daily cocaine (20 mg/kg, i.p.) or saline control injections began on day 1 until day 5 and sensitization in the form of distances traveled was measured on day 20 following a single challenge cocaine injection (10 mg/kg, i.p.). Locomotor activity was measured for the 60 min (control period) immediately prior to injection and the 90 min immediately following the 10 mg/kg challenge cocaine injection (i.p.). (A) Time courses of distances traveled of WT (left panel), ASIC1a−/− (middle panel), and ASIC2−/− (right panel) mice pretreated with saline (open circles) or cocaine (filled triangles) in the 150-min session. The first 60 min denote pre-injection activity. n = 21 to 28 per group. Data shown are the average total distances traveled given in 5-min intervals. (B) Total distances traveled in the total 60-min (left panel), first 15 min (middle panel, from 60 to 75 min) and following 45 min (right panel, from 80 to 120 min) after challenge cocaine injection for WT, ASIC1a−/−, and ASIC2−/− mice. n = 21 to 28 per group. Data shown are the average total distances traveled in the 60-min session after challenge cocaine injection. * p < 0.05; ** p < 0.01; *** p < 0.001; n.s. means not significant. (C) Relative amplitude of total distances traveled in the total 60-min (left panel) and first 15 min (right panel, from 60 to 75 min) session after challenge cocaine injection in WT, ASIC1a−/−, and ASIC2−/− mice. Mice pretreated with saline were used as control subjects. The relative amplitude represents the ratio of the average total distance traveled in 60 or 15 min by mice pretreated with cocaine divided by the average total distance traveled in 60 or 15 min by mice pretreated with saline. ** p < 0.01.; n.s. means not significant. n = 21 to 28 per group. Pre-Saline: mice pretreated with saline; Pre-Cocaine: mice pretreated with cocaine; WT: wild-type mice; ASIC1a KO: ASIC1a knockout mice; ASIC2 KO: ASIC2 knockout mice. Min: minutes.