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. 2013 Dec 5;8(12):e82436. doi: 10.1371/journal.pone.0082436

Figure 5. ERα and Wnt3A synergistically induce ectopic bone formation.

Figure 5

(A) Co-expression of Wnt3A and ERα in MPCs. Subconfluent iMEFs were co-infected with AdWnt3A and AdERα, or AdGFP. Fluorescence signal was examined at 24h post infection. (B) The transduced cells described in (A) were collected and injected into athymic nude mice subcutaneously and intramuscularly. Ectopic bony masses were harvested after 6 weeks. The GFP only and ERα+GFP group did not form any masses during the experimental period. Representative results for subcutaneous masses are shown. (C) H & E staining of the ectopic bony masses. The retrieved masses were decalcified, paraffin-embedded and subjected to H & E staining. Representative images are shown. “b”, osteoid matrix; “c”, injected/undifferentiated cells.