Figure 9. Levels of oxidative burst, H₂O₂ production, and reduced glutathione in wild-type and CatE ^−/− macrophages.

(A) Levels of oxidative burst by macrophages. Macrophages in cell suspension (1 × 10⁷ cells/mL) were incubated with zymosan suspension at 37 °C for 30 min. Levels of oxidative burst of macrophages were determined by a luminophotometer. (B) The levels of H₂O₂ production of macrophages. Macrophages (1 × 10⁶ cells/50 μL) were cultured without any stimulation at 37°C for 1 h. Determination of H₂O₂ production into the culture media by macrophages was performed by an Amplex Redassay kit. (C) The GSH levels of cell lysates of macrophages were assayed with NADPH, measuring the development with DTNB at 412 nm with spectrophotometer. (D) Levels of H₂O₂ in the serum (50 μL) of mice were determined by an Amplex Redassay kit. The data are the means ± SD of values from 4 independent experiments. *P <0.05, **P <0.01, ***P <0.001.