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. Author manuscript; available in PMC: 2013 Dec 7.
Published in final edited form as: Chem Biol. 2011 Sep 23;18(9):10.1016/j.chembiol.2011.06.015. doi: 10.1016/j.chembiol.2011.06.015

Figure 2. CT08 and CT09 differentially inhibit secretory and transmembrane protein expression.

Figure 2

Primary human cells were stimulated under various pro-inflammatory conditions (see Supplemental Information for details) in the presence of increasing concentrations of CT08 or CT09. After 24 h, expression levels of a total of 25 secreted and membrane proteins (see Table S1 for details) were quantified by cellular immunoassays and normalized to DMSO controls (mean ± SEM; n = 3). EC50 values were estimated by curve-fitting with Prism software (for the complete set of dose-response curves and 95% confidence intervals, along with EC50 values grouped by system, see Figure S1). (A) Class I proteins, VCAM and TNFα, are similarly sensitive to CT08 and CT09. (B) Class II proteins, uPAR and E-selectin, show greater sensitivity to CT09 than CT08. (C) Class III proteins, TGFβ1 and MMP-1, show < 60% inhibition by 3.3 μM CT09 or CT08.